| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.016124 |
| Chromosome: | chromosome 6 |
| Location: | 3930150 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278196 | (1 of 3) PF01575 - MaoC like domain (MaoC_dehydratas) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGGCAGCTGTAGTTACACGTGTGTGTGTGTGTGTGTGTGTGTGTGTGT |
| Internal bar code: | GCATAATCTTATATAATTAAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2653 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTCTACCTACAGGCCAT |
| Suggested primer 2: | CACCAAACACAGTCACTGCG |