Insertion junction: CLIP2.016143_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.016143
Chromosome:chromosome 6
Location:4169408
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278233 OPR60 OctotricoPeptide Repeat protein 60 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTCCTGCTTGAGTCTTGCCATGTATTTGCGTGCACATGAGCAAACTTT
Internal bar code:GGCCGCGCGAGGTATTACAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1443
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:14

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGCCGTCCCATTTGACAA
Suggested primer 2:CAACGAGCAGCTGGTAGGAA