Insertion junction: CLIP2.016152_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.016152
Chromosome:chromosome 3
Location:5903428
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g188750 HEL21 (1 of 1) K13177 - ATP-dependent RNA helicase DDX1 [EC:3.6.4.13] (DDX1); DEAD/DEAH box helicase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAAAGACACACATGTAGACACGCAAGCGCACGAATACACACAAGGATA
Internal bar code:AACTGAGGTCTAGCTTGAGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2279
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TCGCTGTTGATCCCTCACAC
Suggested primer 2:AATAGCGCGGTGGTCATAGG