| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.016158 |
| Chromosome: | chromosome 12 |
| Location: | 8675494 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g548000 | (1 of 1) K14550 - U3 small nucleolar RNA-associated protein 10 (UTP10, HEATR1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCCCAAGGGACCATGCACCCACGAGGGGGCGCCAGCGGCGCCCCCGGG |
| Internal bar code: | ATGTGTCTAGGCTTCATGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4491 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGATTGTGTCGGAGCACA |
| Suggested primer 2: | CTGACGTGCGTTGAATAGCG |