Insertion junction: CLIP2.016167_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.016167
Chromosome:chromosome 12
Location:4898173
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g524000 SUC1,GTR7 (1 of 1) 2.4.1.14 - Sucrose-phosphate synthase / UDP-glucose-fructose-phosphate glucosyltransferase; Sucrose-phosphate synthase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGGGGGTGAAGGAAGCACAGCAGCAGCGCATGACATGCGGCGAAGCACG
Internal bar code:AAGTGACTGTGGGTTGTAGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1311
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TGACACGCCTCTCTTGACAC
Suggested primer 2:ATTTACAACCCCCACCCACC