Insertion junction: CLIP2.016207_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.016207
Chromosome:chromosome 16
Location:3799560
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g688550 GSTS1,GST1 (1 of 3) 2.5.1.18//5.3.99.2 - Glutathione transferase / S-(hydroxyalkyl)glutathione lyase // Prostaglandin-D synthase / Prostaglandin-H(2) Delta-isomerase; Glutathione S-transferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGACTTCTGCTCCGGCCAGGTGGGCCCATCGATACGCACGTCCTGTGTT
Internal bar code:ATATGATCATGCTGCTAGATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1714
LEAP-Seq percent confirming:20.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:GTAACCTGGCACCGACATCA
Suggested primer 2:CTACACGCCGACAGATCCTC