Insertion junction: CLIP2.016212_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.016212
Chromosome:chromosome 7
Location:3839426
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g338750 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATCAAATGCTTAGATAGCGCCCTTAGTTTACTGTGCGTGTAACCGTGTA
Internal bar code:GTAGAATGACCTTTGAGGGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2999
LEAP-Seq percent confirming:86.8421
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CTCTTGCTCCCACTGCTGG
Suggested primer 2:TACAGGTGAGTGCCGGAAAC