| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.016217 |
| Chromosome: | chromosome 6 |
| Location: | 2924369 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273200 | (1 of 1) K03762 - MFS transporter, MHS family, proline/betaine transporter (proP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGCTGACAGGGTCACGCAGGTGCCTGAGGGGCGAGGGACAACAGCAAC |
| Internal bar code: | GAAAGGATGTGGACGTGGGCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3276 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTACACTGCTGCTCACTGCT |
| Suggested primer 2: | GAATGGGATGGAGGGTGGTG |