Insertion junction: CLIP2.016219_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.016219
Chromosome:chromosome 12
Location:7816158
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g553900 CNG2 (1 of 1) K04905 - potassium voltage-gated channel Eag-related subfamily H member 2 (KCNH2); Cyclic-nucleotide gated potassium channel intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAAACATACACTGACGGACGACGAAACCCGTGGCTTCGCTGTCTCCGCTT
Internal bar code:GGGACGCACGTATTTTAAGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1068
LEAP-Seq percent confirming:89.4737
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTCCTCGCTCTTTTGCAT
Suggested primer 2:TTCAATGTGGCGACGGAGAA