Insertion junction: CLIP2.016235_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.016235
Chromosome:chromosome 12
Location:5938967
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g533800 CYN22 Cyclophilin 22; (1 of 1) K09567 - peptidyl-prolyl isomerase H (cyclophilin H) (PPIH, CYPH) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGTGTCGTGGGCCCCGTACATGCCCCTGTGCCCCGACAACGCTGCCCT
Internal bar code:TATACAGGTATGGTCGCTGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:3648
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:60
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:60

Suggested primers for confirmation by PCR

Suggested primer 1:TCCGACAACACTGGTAAGCC
Suggested primer 2:GGTCAGCTGGGAGGTCATTC