Insertion junction: CLIP2.016235_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.016235
Chromosome:chromosome 12
Location:5938969
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g533800 CYN22 Cyclophilin 22; (1 of 1) K09567 - peptidyl-prolyl isomerase H (cyclophilin H) (PPIH, CYPH) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCAAGCGCGGCACCGCACGCATACACGGTAACACGGTAACACACGCA
Internal bar code:TATACAGGTATGGTCGCTGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1910
LEAP-Seq percent confirming:55.1724
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:13
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GGTCAGCTGGGAGGTCATTC
Suggested primer 2:TCCGACAACACTGGTAAGCC