Insertion junction: CLIP2.016238_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.016238
Chromosome:chromosome 9
Location:3217009
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g394658 LCI28 (1 of 1) 1.1.1.21 - Aldehyde reductase / Polyol dehydrogenase (NADP(+)); Low-CO2-induced aldose reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGACACACGACCCCTAAGGCAGTGGCCCGAAGTTCTCAACCGACGCTT
Internal bar code:GTTGACTGGATTGGTAAAGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:599
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TCCAGATAGGACACCTGGGG
Suggested primer 2:ACCAGAATGAGCACGAGGTG