| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.016248 |
| Chromosome: | chromosome 14 |
| Location: | 1558845 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g618150 | (1 of 1) PF07058 - Microtubule-associated protein 70 (MAP70) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACACCACAAGGACGGTACGGTATGAAGCACGGCGGGGCAAGCGCTGGGG |
| Internal bar code: | AATTTCCGTGGAGTCTTTCGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4452 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 107 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 107 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTGCGGGTTTACTCTGA |
| Suggested primer 2: | CAGAACCCTTGCCGTGGTAT |