Insertion junction: CLIP2.016262_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.016262
Chromosome:chromosome 6
Location:8378019
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g307800 CYN49 (1 of 1) IPR003609//IPR029000 - PAN/Apple domain // Cyclophilin-like domain; Cyclophilin 49 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACAAGGAAAACATTACGCGTAGGACAGTGTATGCGATGCAGAGTTACGG
Internal bar code:GACTATCTAAACATTACTAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:108
LEAP-Seq percent confirming:60.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCTGTATGCCAAAGTCCT
Suggested primer 2:AGGAAGCGGAAACTTGAGGG