| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.016263 |
| Chromosome: | chromosome 3 |
| Location: | 5274150 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182400 | (1 of 7) K18932 - palmitoyltransferase (ZDHHC) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACGTGGTGCAGCTGTGGGCACGCTGCTACCTCCTGCGGCCGCCCCTGA |
| Internal bar code: | TATCGTCTAAGCCATCTAGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1935 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCAAGCTGACCCCCACTAC |
| Suggested primer 2: | CTTCTTCGCGCTACTGGCTA |