| Insertion cassette: | CIB2 | 
| Side of cassette: | 3' | 
| Strand: | + | 
| Strain: | CLIP2.016276 | 
| Chromosome: | chromosome 2 | 
| Location: | 1377203 | 
| Confidence (%): | 80 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| Cre02.g083100 | PRL1 | (1 of 14) PTHR10334 - CYSTEINE-RICH SECRETORY PROTEIN-RELATED; Predicted extracellular protein | intron | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTGGTGCTCGAAGCGGCAGTTGCCGGCCCAGGCGGCGGCGCTAGCCTC | 
| Internal bar code: | ATTGCGATTTATGCCTTGCAGG | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4263 | 
| LEAP-Seq percent confirming: | 100.0 | 
| LEAP-Seq n confirming: | 52 | 
| LEAP-Seq n nonconfirming: | 0 | 
| LEAP-Seq n unique pos: | 52 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGAGCTTGCTTGCCTTACT | 
| Suggested primer 2: | CCTAATCGCACACTGCATGC |