| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.016295 |
| Chromosome: | chromosome 10 |
| Location: | 3021528 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g440650 | (1 of 1) PTHR12176:SF18 - METHYLTRANSFERASE-LIKE PROTEIN 13 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGCACCGACTGGATGGTGATGGACGCACGGCGCCTGGCGTTTCGCAGAT |
| Internal bar code: | GGTCCGCTGCTGATGGCGTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5140 |
| LEAP-Seq percent confirming: | 99.2308 |
| LEAP-Seq n confirming: | 129 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 130 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCGCTCCTCCTTATCTG |
| Suggested primer 2: | GTACTGGGATGCGCGATACA |