Insertion junction: CLIP2.016298_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.016298
Chromosome:chromosome 17
Location:1344654
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g705778 Chlamydomonas-specific family W protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGTGAGAGCGGGGGGAGGCCGGCCGGGAGGAAAGACACGTGCCCTTCC
Internal bar code:TTGTCGGAGGTGTGATTATCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1723
LEAP-Seq percent confirming:72.2222
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:AGACCAGCGAGTTCCTTTCG
Suggested primer 2:GAAACAAACAGTGCGCGAGT