| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.016298 |
| Chromosome: | chromosome 17 |
| Location: | 1344654 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g705778 | Chlamydomonas-specific family W protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGGTGAGAGCGGGGGGAGGCCGGCCGGGAGGAAAGACACGTGCCCTTCC |
| Internal bar code: | TTGTCGGAGGTGTGATTATCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1723 |
| LEAP-Seq percent confirming: | 72.2222 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACCAGCGAGTTCCTTTCG |
| Suggested primer 2: | GAAACAAACAGTGCGCGAGT |