| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.016309 |
| Chromosome: | chromosome 2 |
| Location: | 650988 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g077750 | FAP211,CSE12 | Flagellar Associated Protein 211 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGTGACGGAGGCTCGGTCCGTGTCAGCAGATGGGGAAAACTGAATGCG |
| Internal bar code: | ATGTTTTTCCTTCGGGGATTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 62.5 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACGGTTGTCAAGCTGTG |
| Suggested primer 2: | TGGTCGTTGAGGACTTCACG |