| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.016333 |
| Chromosome: | chromosome 16 |
| Location: | 2037795 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g656950 | (1 of 1) PTHR13140:SF300 - PROTEIN TAG-278-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACCAGCGACACCAGCCGGCTGTCCACGTCGGCCTTGGAGGTGCCAGC |
| Internal bar code: | GCGTTAGCGGGTTGGACCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5840 |
| LEAP-Seq percent confirming: | 83.6066 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAGGAGGCGCTTCAAAGT |
| Suggested primer 2: | CTCCGACTCCAGAGACTCCA |