Insertion junction: CLIP2.016337_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.016337
Chromosome:chromosome 2
Location:4114003
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g103500 (1 of 1) 1.14.13.169 - Transferred entry: 1.14.18.5 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTCTCGCAGCCGTTCTTCACGCACTGGGACTGGCTGCTGGGCACCTTC
Internal bar code:TTCGGGCGAGACGTCTGCAATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2810
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTAATGCTACGTTGCCGC
Suggested primer 2:TCGTGGTGGACAAGGACATG