| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.016351 |
| Chromosome: | chromosome 17 |
| Location: | 3628659 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g725350 | PSL4,GLC2B | Glucosidase IIb; (1 of 1) K08288 - protein kinase C substrate 80K-H (PRKCSH) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCGGGTCGGATGGGCACGCTGAGCGTACCCTGCAGGAAGAGGGCCAAC |
| Internal bar code: | GATCCTCTGGGACCCACTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 615 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCCTCAGGATCGTTAGTC |
| Suggested primer 2: | AGCGTGTGGAACCGAAAGAT |