Insertion junction: CLIP2.016359_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.016359
Chromosome:chromosome 7
Location:476845
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g315550 (1 of 2) IPR005654//IPR027417 - ATPase, AFG1-like // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGAAGCACCTGTTACTAACCTTGGGTGGGGATTGGTGTCCACACGTGTG
Internal bar code:AGTCTTGTCTTTGCATATGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:128
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GCCTTGTTGTTCACCACGAC
Suggested primer 2:TGCTAGGACTGTTGGCATGG