| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.016360 |
| Chromosome: | chromosome 10 |
| Location: | 868589 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g423600 | (1 of 1) PTHR10891//PTHR10891:SF5//PTHR22847 - EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN // SUBFAMILY NOT NAMED // WD40 REPEAT PROTEIN | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCCTTCCGCCCTCAATCCTTCCCCGGTACCAGTCTTCCTAAGCAGCCCT |
| Internal bar code: | AAAGATGGCTTTGAGCCAATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2178 |
| LEAP-Seq percent confirming: | 97.2973 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCACAACCACAACCACCAT |
| Suggested primer 2: | CTTGAGAGCGTGTGGACACT |