Insertion junction: CLIP2.016361_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.016361
Chromosome:chromosome 9
Location:5716487
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g410500 PAP11 (1 of 1) IPR002934//IPR023214 - Polymerase, nucleotidyl transferase domain // HAD-like domain; Class-II RNA nucleotidyl transferase 11 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCAGAACCAGTGCCGCACACTTTCTGTTCCACACGTGATGCGCCTCAG
Internal bar code:ACTTTAAAATTGGGTTAAGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:678
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:27
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:27

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTGCTCGAGCCCAATGAA
Suggested primer 2:CGCTCCTGATCTAGCTGCTG