Insertion junction: CLIP2.016387_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.016387
Chromosome:chromosome 6
Location:5636170
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g287650 (1 of 1) 2.1.1.43//6.3.2.19 - Histone-lysine N-methyltransferase / Protein-lysine N-methyltransferase // Transferred entry: 2.3.2.23, 2.3.2.27 and 6.2.1.45 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGATCGCGTGTGGTTGCGTTTGTGCGCAAATGGCTGTGCTGGTGTGTGTG
Internal bar code:ATAATGGAATGCATTTCCTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1914
LEAP-Seq percent confirming:90.0
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCGGCGGTTTATAAAAGG
Suggested primer 2:CTCCTCAGGCATAGTGCTCG