Insertion junction: CLIP2.016392_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.016392
Chromosome:chromosome 12
Location:9833185
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g546452 GT90F45,GT90-45 (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90); GT90 family protein 45 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACTGAAGCAGTGGGACGGCAAGAACCGCGGCAAGACGGTGCGTTGCC
Internal bar code:AAGAGGGTTCAACATTGGACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:306
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGGATGATAGGCACGACGAG
Suggested primer 2:ACGTCCGCACTGAAAGATGT