| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.016398 |
| Chromosome: | chromosome 7 |
| Location: | 1288874 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g322176 | (1 of 1) K03002 - DNA-directed RNA polymerase I subunit RPA2 (RPA2, POLR1B) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGCATGGAATCGACTCCCCCCCCTGCCCTGCCCACCCACCCACTTAC |
| Internal bar code: | TAGGACTATGGCACGGGTTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 49 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCACCCCTTGTTATGGCAT |
| Suggested primer 2: | TCAAGCCAAACTAGCGAGCA |