Insertion junction: CLIP2.016412_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.016412
Chromosome:chromosome 16
Location:6858589
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g671700 (1 of 4) PTHR11920//PTHR11920:SF248 - ADENYLATE AND GUANYLATE CYCLASES // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAACACGTTCGCCATGCCCAGCCACCTTGAAGCCCCGTCTGGCTCCTG
Internal bar code:CTTCAGGCTGAGAATTCCGAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:560
LEAP-Seq percent confirming:52.9412
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GTAAGGAAACAGGTCGGGGG
Suggested primer 2:CGATTACAATGGCGTGGCTG