Insertion junction: CLIP2.016417_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.016417
Chromosome:chromosome 2
Location:7638103
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g147750 FAP209 Flagellar Associated Protein 209 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGCCCCTGGCGCTGCCGGGCAGTGGTGTGCCGGCGCCCTCGCGACCCC
Internal bar code:CAACGTAAATGTTGTGATATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:2731
LEAP-Seq percent confirming:98.6487
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:CGGACTACGGAGATCTGCAC
Suggested primer 2:TGCTGACGGAAGACTTGCAT