| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.016419 |
| Chromosome: | chromosome 16 |
| Location: | 7362666 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679557 | FAS5 | (1 of 10) PTHR10900 - PERIOSTIN-RELATED; Fasciclin-like protein 5 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCATACACAGCAGGGTGCTCCGTTGGGACAACCGCAGCTGCCGGGCGTC |
| Internal bar code: | CATTGTTCCGGAGTTGCTCGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2067 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCGTTGAACAAGTCCAC |
| Suggested primer 2: | TCCGATGAACACCTTCGTGG |