| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.016423 |
| Chromosome: | chromosome 9 |
| Location: | 1746817 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398350 | (1 of 2) K10597 - ubiquitin conjugation factor E4 B (UBE4B, UFD2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGTGCTGCGGTCTGCGGCGGCCTGTGCTCCGATGCAGTTAGTACCGAAG |
| Internal bar code: | CCCAATGTTTAAGTTAATTAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3240 |
| LEAP-Seq percent confirming: | 15.5844 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGGACCGATGACTGCACTG |
| Suggested primer 2: | GCATTCTTGTGCACGGGTTT |