Insertion junction: CLIP2.016442_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.016442
Chromosome:chromosome 10
Location:4747229
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g452550 HOPP1,HOP1 Homologous-pairing protein 1; (1 of 3) PF02301 - HORMA domain (HORMA) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCAATACCGCGGCCTCCTGGTCTCCTGCTGCTCACCTCCGCCGGCACC
Internal bar code:CCAACATACAATGGCCGAGTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1485
LEAP-Seq percent confirming:30.7692
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:9
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:CGGTAGATGGGAATGGACGG
Suggested primer 2:TACCATCCCTGCTGTGCTTG