| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.016444 |
| Chromosome: | chromosome 15 |
| Location: | 186693 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g634701 | DIV20,RECQ4 | (1 of 1) K10730 - ATP-dependent DNA helicase Q4 [EC:3.6.4.12] (RECQL4); RecQ-class helicase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAATCCCGCCAGCTGCGCCAACTGCCGCAGAGCCTGAGAGCTGCTCAT |
| Internal bar code: | TTCATTCTAATAGAATTCCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 217 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCCTCCTCCTCTTTCGG |
| Suggested primer 2: | GTGGATGGTGGGATGGTAGC |