| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.016447 |
| Chromosome: | chromosome 6 |
| Location: | 4172132 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278234 | FAP343 | (1 of 19) PF00397 - WW domain (WW); Flagellar Associated Protein 343 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCACCACCGCCCTGGTATGGCCCCCTGCAGGCCAAGGCTGAGCAGGA |
| Internal bar code: | ATCAGTTGAAGTGATCCAGTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2350 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGATACAGCGTGTGTCA |
| Suggested primer 2: | CTCGCACACACCAATCTTGC |