Insertion junction: CLIP2.016453_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.016453
Chromosome:chromosome 10
Location:409270
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g420600 (1 of 2) PTHR22840//PTHR22840:SF12 - FAMILY NOT NAMED // WD REPEAT-CONTAINING PROTEIN 36 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTACGTCTCCCTTGTTTACTCTTGCGACTGGCACACGGCACAGCCCCC
Internal bar code:GGTATTTATCAACTTGCCAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1988
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:19
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:CACAAGCAAACTGACACCCG
Suggested primer 2:CGACACCACCTTCACACGTA