| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.016490 |
| Chromosome: | chromosome 2 |
| Location: | 7855182 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g146050 | ATO2 | (1 of 1) 2.3.1.9 - Acetyl-CoA C-acetyltransferase / Acetoacetyl-CoA thiolase; Acetyl-CoA acyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGCCCACTCGCACCTCCCAAAGCCGCCGCCAAGGACGCACACGCCGTC |
| Internal bar code: | AGGCCACTTCCCACACACCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2102 |
| LEAP-Seq percent confirming: | 97.9592 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGAGTTTGGAGGGGACTGG |
| Suggested primer 2: | ACATGGTGGAATGGAGGCTG |