| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.016519 |
| Chromosome: | chromosome 17 |
| Location: | 1815050 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g709250 | (1 of 1) PTHR23137:SF3 - VESICLE TRANSPORT PROTEIN SFT2C | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATGAATTAAGGAGTCGTTGAGGTGCCCTCGCAGCCTGCCGACCCCCGC |
| Internal bar code: | GCGTGTGGCGCTGTATTGTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5172 |
| LEAP-Seq percent confirming: | 99.5536 |
| LEAP-Seq n confirming: | 223 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 224 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGCCGACTAGGACATAC |
| Suggested primer 2: | TGTTAGCCGACTGGAACACC |