| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.016521 |
| Chromosome: | chromosome 1 |
| Location: | 4642004 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032100 | (1 of 1) K10570 - DNA excision repair protein ERCC-8 (ERCC8, CKN1, CSA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGGCATTGTCACCCCAGCTGCACGCTGGGCGGCATTTTTGGGGAACG |
| Internal bar code: | GAGTCCGTGCACTCGGGGCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6330 |
| LEAP-Seq percent confirming: | 77.686 |
| LEAP-Seq n confirming: | 94 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 121 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGTGCGGTTTGCTGTACGT |
| Suggested primer 2: | CTTCCACAACCGTGCAACAG |