Insertion junction: CLIP2.016524_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.016524
Chromosome:chromosome 2
Location:2193960
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g089608 (1 of 1) K11136 - regulator of telomere elongation helicase 1 [EC:3.6.4.12] (RTEL1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGGAGAGGGAGAGGGACGCTGCGCGCTGCATGTTGATGCTCAAAAGCAC
Internal bar code:ATGGCGTTAGGTTATAAGTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:184
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGAAGAGCCGGGATGAGGTA
Suggested primer 2:GTGACTAGGTGGGTGGTTGG