Insertion junction: CLIP2.016545_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.016545
Chromosome:chromosome 5
Location:984615
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g247100 COPT1,COTH1 spore coat protein H; (1 of 3) PF08757 - CotH protein (CotH) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCTTCAGGGACCGGACGGCAAGCAGTCTCAGGAGGTGTCGCTGCTTGG
Internal bar code:TCGAGACGCTGTATATAAATAC

Confirmation - LEAP-Seq

LEAP-Seq distance:624
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACGACTTCCCAGTTCCC
Suggested primer 2:TTCGCAGTGACAAAGCAACG