| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.016553 |
| Chromosome: | chromosome 11 |
| Location: | 2217571 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467721 | (1 of 1) IPR027446 - Variant surface glycoprotein C-terminal domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTAAATGCTGTCCACGGCGGCGCCACAGCGTGGTGGCCGCTGGCGGGTG |
| Internal bar code: | AGGGTTTGGCTATACCCGTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2068 |
| LEAP-Seq percent confirming: | 95.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCAACGTTTACCGACCCT |
| Suggested primer 2: | GATAGAGAGCGTGGGAAGCC |