Insertion junction: CLIP2.016569_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.016569
Chromosome:chromosome 17
Location:709652
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g700700 (1 of 37) IPR008979 - Galactose-binding domain-like intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCCGGCGCCGCTTGGGCGGGCTTCGTTTTGTTGTTTTTTTCTAACACA
Internal bar code:TATACCTCCTCAGGCAGCCGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1341
LEAP-Seq percent confirming:76.9231
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACCGCAACGTCAAATG
Suggested primer 2:CGTCGCGAGGTACTTGATCA