| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.016594 |
| Chromosome: | chromosome 7 |
| Location: | 1768622 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g325722 | (1 of 1) PTHR13215:SF0 - SUB1 HOMOLOG (S. CEREVISIAE); RNA polymerase coactivator | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACATACAGATACACACACACACACGCGCAACGTTTTCCTTGTGCTCTTT |
| Internal bar code: | CTCAAAGCGTGATATCTCGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1001 |
| LEAP-Seq percent confirming: | 60.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCAGCAAACCGCAAGAATC |
| Suggested primer 2: | CAAACGCGACATGGGCTATG |