Insertion junction: CLIP2.016604_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.016604
Chromosome:chromosome 2
Location:7874833
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g145850 FAP112 Flagellar Associated Protein 112; (1 of 3) IPR006130 - Aspartate/ornithine carbamoyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTACAGGTTCACTGCAGGAACACAACAAACAAGCAGTGTTCCGCTAGTAA
Internal bar code:ACCTAGGAATTGGCCCATGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1094
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CATATTGCCCGCAGTTGAGC
Suggested primer 2:CAAGCGCGTTGTTGAACTCA