Insertion junction: CLIP2.016606_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.016606
Chromosome:chromosome 8
Location:2608153
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g373200 FXL7 FixL-like PAS domain protein; (1 of 11) PF13426 - PAS domain (PAS_9) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTATTAGCCCTGACCCTGCCGGCGCGGGTGCGCCCACCAAGTCGTGAGT
Internal bar code:GCCGCTGCCAGTCTGCCGTCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2155
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:GCTACGCTCGATCTGCTTCT
Suggested primer 2:CAATGTGTGCGGCAGAAACA