Insertion junction: CLIP2.016616_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.016616
Chromosome:chromosome 16
Location:2659666
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g661500 (1 of 4) 3.4.21.19 - Glutamyl endopeptidase / V8 proteinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAACGCAATGGAACGGCGCTGCAGTTATCAACCAGAACACCTTCAGCTTC
Internal bar code:TTGAGTTCCGCTCGTTTGTTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3799
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:109
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:109

Suggested primers for confirmation by PCR

Suggested primer 1:ACCTCGACGCACTTCCAAAT
Suggested primer 2:TGACTGTCTCGCAATGGCTT