| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.016655 |
| Chromosome: | chromosome 4 |
| Location: | 1273625 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g214850 | (1 of 1) IPR000104//IPR000253 - Antifreeze protein, type I // Forkhead-associated (FHA) domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGGGGAGAAGGAGCCGGGCGGCCGGGAGGCGCGGGAGGAGGCGCGGGA |
| Internal bar code: | GGTTAGCCATTTCATGGTCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3281 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGCACATGAATACCTGC |
| Suggested primer 2: | AGCAGGAGGAAGAGGAGGAG |