Insertion junction: CLIP2.016657_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.016657
Chromosome:chromosome 6
Location:2846517
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g272150 (1 of 1) 1.8.4.2 - Protein-disulfide reductase (glutathione) / Insulin reductase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGCTGTTCATAAACTCGCGACAATCCGCGCACGAGCGTGAGCTCAGCAC
Internal bar code:TTGCATTTGTACTTTGATATGT

Confirmation - LEAP-Seq

LEAP-Seq distance:2908
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:53

Suggested primers for confirmation by PCR

Suggested primer 1:CTTATGTGCGCACGTTCGAG
Suggested primer 2:GTCACCCAATCACTCCCCTG