| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.016662 |
| Chromosome: | chromosome 11 |
| Location: | 222790 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467554 | (1 of 15) PF01694 - Rhomboid family (Rhomboid) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCGTGAGAGCCGCCCCGCTGGCAGGGGTGTTTGCCCGGAGCTTGCAGCT |
| Internal bar code: | GGCCGTTTCACCTTTTTCTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 574 |
| LEAP-Seq percent confirming: | 73.6842 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGAGTGGGGAGGTGGAT |
| Suggested primer 2: | CCTGATAACGACGACCGGAG |